Generation of nine iPSC lines (HNMUi002-A, HNMUi003-A, HNMUi004-A, HNMUi005-A, HNMUi006-A, HNMUi007-A, HNMUi008-A, HNMUi009-A, HNMUi010-A) from three Chinese families with thalassemia

Ping Long; Zhen Wang; Huamei Yang; Zheng Liu; Bangyong Wu; Gaobu Zhong; Jingxi Chen; Chao Sun; Fei Wang; Yao Zhou; Fei Sun; Qi Li; Yanlin Ma

doi:10.1016/j.scr.2020.102014

Generation of nine iPSC lines (HNMUi002-A, HNMUi003-A, HNMUi004-A, HNMUi005-A, HNMUi006-A, HNMUi007-A, HNMUi008-A, HNMUi009-A, HNMUi010-A) from three Chinese families with thalassemia

Stem Cell Res. 2020 Dec:49:102014. doi: 10.1016/j.scr.2020.102014. Epub 2020 Oct 1.

Authors

Ping Long¹, Zhen Wang¹, Huamei Yang¹, Zheng Liu², Bangyong Wu¹, Gaobu Zhong¹, Jingxi Chen¹, Chao Sun¹, Fei Wang¹, Yao Zhou¹, Fei Sun¹, Qi Li³, Yanlin Ma⁴

Affiliations

¹ Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China; Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, China; Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China.
² College of Medical Laboratory Science, Guilin Medical University, Guilin, Guangxi, China.
³ Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China; Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, China; Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China. Electronic address: liqi1970@hotmail.com.
⁴ Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China; Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, China; Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China. Electronic address: mayanlinma@hotmail.com.

PMID: 33039806
DOI: 10.1016/j.scr.2020.102014

Abstract

Thalassemia is a group of single-gene recessive inherited hemoglobin disorders caused by a mutation or deletion of one or more globin genes, which results in abnormal globin chain synthesis and hemoglobin formation. In this study, human iPSC lines HNMUi002-A, HNMUi003-A, HNMUi004-A, HNMUi005-A, HNMUi006-A, HNMUi007-A, HNMUi008-A, HNMUi009-A, HNMUi010-A were generated from the amniotic fluid cells or urine-derived cells isolated from 9 patients with thalassemia. The iPSC lines exhibited the normal karyotype, expressed pluripotency markers, and carried α- or β- globin gene mutations. These pluripotent stem cell lines will serve as useful tools for studying pathophysiological mechanism of thalassemia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

China
Humans
Induced Pluripotent Stem Cells*
Mutation
Pluripotent Stem Cells*
Thalassemia*