Isolation of the Candida tropicalis gene for P450 lanosterol demethylase and its expression in Saccharomyces cerevisiae

Biochem Biophys Res Commun. 1987 Aug 14;146(3):1311-7. doi: 10.1016/0006-291x(87)90792-3.

Abstract

We have isolated the gene for cytochrome P450 lanosterol 14 alpha-demethylase (14DM) from the yeast Candida tropicalis. This was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. Identity of this gene was confirmed by a) observing a heme binding region common to all P450s after sequencing the 3' portion of the gene, and b) based upon tests of its expression in strains of Saccharomyces cerevisiae.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Candida / enzymology
  • Candida / genetics*
  • Cytochrome P-450 Enzyme System / genetics*
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • Drug Resistance
  • Escherichia coli / genetics
  • Genes*
  • Genes, Fungal*
  • Ketoconazole / pharmacology
  • Oxidoreductases / genetics*
  • Plasmids
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics
  • Sterol 14-Demethylase

Substances

  • DNA, Fungal
  • Cytochrome P-450 Enzyme System
  • Oxidoreductases
  • Sterol 14-Demethylase
  • Ketoconazole

Associated data

  • GENBANK/M17595