Protocol for High-Throughput Screening of Neural Cell or Brain Tissue Protein Using a Dot-Blot Technique with Near-Infrared Imaging

STAR Protoc. 2020 Sep 18;1(2):100054. doi: 10.1016/j.xpro.2020.100054. Epub 2020 Jun 13.


Dot blotting allows for the rapid screening of a larger number of samples and/or targets than more traditional methods, such as a western blot or in-tissue-based methods. We have developed a dot-blot assay specifically for use with a LiCor Odyssey CLx imager, which allows for sensitive detection of proteins in the infrared range. Here, we provide a detailed protocol for the preparation of brain tissue and neural cell culture lysates for analysis of protein targets by dot blotting.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain* / cytology
  • Brain* / metabolism
  • Cell Culture Techniques / methods
  • Cells, Cultured
  • High-Throughput Screening Assays / methods*
  • Humans
  • Immunoblotting / methods*
  • Mice
  • Neurons* / chemistry
  • Neurons* / cytology
  • Spectroscopy, Near-Infrared / methods*