MiR-92b-3p inhibits proliferation and migration of C2C12 cells

Cell Cycle. 2020 Nov;19(21):2906-2917. doi: 10.1080/15384101.2020.1827511. Epub 2020 Oct 12.

Abstract

Skeletal muscle, a critical component of the mammalian body, is essential for normal body movement. miRNAs are well documented in gene post-transcription regulation in many biological processes, including muscle development and maintenance. miR-92b-3p, which is often associated with tumorigenesis, has never been explored in myoblast development. Here, we used murine-derived C2C12 myoblasts to explore the potential functions of miR-92b-3p in skeletal muscle development. Our results demonstrated that miR-92b-3p mimics inhibited C2C12 cell proliferation and migration, whereas miR-92b-3p inhibitor promoted C2C12 cell proliferation and migration. C2C12 cell differentiation was not affected by miR-92b-3p mimics, according to immunofluorescence and qPCR results. Serum- and glucocorticoid-induced kinase 3 (SGK3) was predicted and validated as a target of miR-92b-3p. Overexpression of SGK3 promoted C2C12 cell proliferation. SGK3 and miR-92b-3p formed a regulatory pathway to modulate C2C12 cell proliferation. In conclusion, miR-92b-3p inhibited C2C12 cell proliferation by targeting SGK3 and impeded C2C12 cell migration.

Keywords: C2C12; SGK3; miR-92b-3p; migration; proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

Grant support

This work was supported by the Guangdong Provincial Key Area Research and Development Program (2018B020203002); The South China Agricultural University Major Project for International Science and Technology Cooperation Cultivation (2019SCAUGH01).