The effects of three recombinant growth factors, IL-3, GM-CSF, and G-CSF, on the blast cells of acute myeloblastic leukemia maintained in short-term suspension culture

Blood. 1987 Sep;70(3):657-63.


The blast stem cells of acute myeloblastic leukemia (AML) respond in cell culture to growth factors by both self-renewal and terminal divisions. Both of these functions have been shown to be stimulated by the recombinant growth factors granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF). In this paper, recombinant gibbon interleukin-3 (IL-3), homologous to human IL-3, was tested on blast cells and compared with the effects of GM-CSF, G-CSF, and medium conditioned by the bladder cell line 5637 (5637-CM). We found that IL-3 was an effective stimulator of blast renewal and terminal divisions. However, great patient-to-patient variation was found. A graphic method of presenting complex comparisons between growth factors is also included.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Blood Cells / drug effects*
  • Blood Cells / pathology
  • Bone Marrow / drug effects
  • Bone Marrow Cells
  • Cell Count
  • Cells, Cultured
  • Cytological Techniques
  • Humans
  • Interleukin-3 / pharmacology*
  • Leukemia, Myeloid, Acute / blood*
  • Methylcellulose
  • Recombinant Proteins / pharmacology*
  • Recombination, Genetic
  • Tumor Stem Cell Assay


  • Interleukin-3
  • Recombinant Proteins
  • Methylcellulose