LncRNA TUG1 regulates the balance of HuR and miR-29b-3p and inhibits intestinal epithelial cell apoptosis in a mouse model of ulcerative colitis

Hum Cell. 2021 Jan;34(1):37-48. doi: 10.1007/s13577-020-00428-5. Epub 2020 Oct 12.


This study aimed to investigate the role of long non-coding RNA (lncRNA) taurine up-regulated 1 (TUG1) in the development of ulcerative colitis (UC) and to explore the underlying mechanisms. A murine model of UC was induced by dextran sodium sulfate (DSS) exposure. The colonic epithelial YAMC cells were treated with TNF-α to simulate the inflammatory environment of intestinal epithelial cells (IECs). RNA pull-down and RIP assays were performed to analyze the interaction between TUG1 and HuR. Luciferase activity assay was conducted to evaluate the interaction between TUG1 and miR-29b-3p. Cell proliferation was evaluated by MTT assay. Cell apoptosis was assessed by flow cytometry and western blot analysis of apoptosis-related proteins. TUG1 overexpression promoted cell proliferation and inhibited cell apoptosis in the TNF-α-stimulated YAMC cells. The mechanistic analysis showed that TUG1 positively regulated the HuR/c-myc axis via its interaction with HuR, leading to upregulation of c-myc expression; meanwhile, TUG1 negatively regulated the miR-29b-3p/CDK2 signaling via binding to miR-29b-3p, leading to derepression of CDK2 expression. Further animal experiments showed that TUG1 overexpression attenuated UC progression in the DSS-induced UC in mice. Collectively, TUG1 inhibits IEC apoptosis and UC progression by regulating the balance of HuR and miR-29b-3p.

Keywords: HuR; TUG1; Ulcerative colitis; miR-29b-3p.

MeSH terms

  • Animals
  • Apoptosis / genetics*
  • Cell Line
  • Colitis, Ulcerative / genetics*
  • Colitis, Ulcerative / pathology*
  • Disease Models, Animal
  • Disease Progression
  • ELAV-Like Protein 1 / genetics*
  • ELAV-Like Protein 1 / metabolism*
  • Epithelial Cells / metabolism*
  • Epithelial Cells / pathology*
  • Gene Expression Regulation, Developmental / genetics*
  • Intestinal Mucosa / cytology*
  • Intestinal Mucosa / metabolism*
  • Intestinal Mucosa / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism*
  • RNA, Long Noncoding / physiology*


  • ELAV-Like Protein 1
  • Elavl1 protein, mouse
  • MIRN29 microRNA, mouse
  • MicroRNAs
  • RNA, Long Noncoding
  • TUG1 long noncoding RNA, human