The role of hydroxylamine in promoting conversion from complete nitrification to partial nitrification: NO toxicity inhibition and its characteristics

Abstract

This study investigated a strategy for hydroxylamine (NH₂OH) addition for promoting the conversion of complete nitrification to partial nitrification in a sequencing batch reactor (SBR). The results showed that continuous dosing of 5 mg-N/L NH₂OH into a complete nitrification reactor for 16 days led to an increase in the nitrite accumulation ratio (NAR) from 0.22% to 95.08% and a significant enhancement in the accumulation of NO and N₂O in the liquid. The maximum concentration of NO in each cycle rose with the increase of NAR during NH₂OH addition. With the stopping of NH₂OH addition, the partial nitrification disappeared progressively in 21 days. The analysis for microbial community showed that Nitrospira was the main NOB and its relative abundance decreased with NH₂OH addition and recovered after the cessation of NH₂OH addition. Accordingly, NH₂OH has a significant and reversible inhibition on Nitrospira and its essence might be related to NO toxicity.

Keywords: Hydroxylamine (NH(2)OH); N(2)O; NO; Nitrite-oxidizing bacteria (NOB); Partial nitrification.