Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion

FEBS Lett. 1987 Aug 31;221(1):167-71. doi: 10.1016/0014-5793(87)80373-3.


A new beta-lactam-inducible penicillin-binding protein (PBP) that has extremely low affinity to penicillin and most other beta-lactam antibiotics has been widely found in highly beta-lactam(methicillin)-resistant Staphylococcus aureus (MRSA). The gene for this protein was sequenced and the nucleotide sequence in its promoter and close upstream area was found to show close similarity with that of staphylococcal penicillinase, while the amino acid sequence over a wide range of the molecule was found to be similar to those of two PBPs of Escherichia coli, the shape-determining protein (PBP 2) and septum-forming one (PBP 3). Probably the MRSA PBP (Mr 76462) evolved by recombination of two genes: an inducible type I penicillinase gene and a PBP gene of a bacterium, causing the formation of a beta-lactam-inducible MRSA PBP.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins*
  • Base Composition
  • Base Sequence
  • Carrier Proteins / genetics*
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Genes, Regulator
  • Hexosyltransferases*
  • Methicillin*
  • Muramoylpentapeptide Carboxypeptidase / genetics*
  • Nucleic Acid Hybridization
  • Penicillin Resistance*
  • Penicillin-Binding Proteins
  • Penicillinase / genetics
  • Peptidyl Transferases*
  • Promoter Regions, Genetic
  • Recombination, Genetic*
  • Sequence Homology, Nucleic Acid
  • Staphylococcus aureus / genetics*


  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carrier Proteins
  • DNA, Bacterial
  • Penicillin-Binding Proteins
  • Peptidyl Transferases
  • Hexosyltransferases
  • Muramoylpentapeptide Carboxypeptidase
  • Penicillinase
  • Methicillin

Associated data

  • GENBANK/Y00688