A Novel bla CTX-M-65-Harboring IncHI2 Plasmid pE648CTX-M-65 Isolated from a Clinical Extensively-Drug-Resistant Escherichia coli ST648

Yang Lü; Haiquan Kang; Jianming Fan

doi:10.2147/IDR.S269766

A Novel bla _CTX-M-65-Harboring IncHI2 Plasmid pE648CTX-M-65 Isolated from a Clinical Extensively-Drug-Resistant Escherichia coli ST648

Infect Drug Resist. 2020 Sep 30:13:3383-3391. doi: 10.2147/IDR.S269766. eCollection 2020.

Authors

Yang Lü¹, Haiquan Kang², Jianming Fan³

Affiliations

¹ State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
² Department of Laboratory Medicine, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, People's Republic of China.
³ The Laboratory of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China.

Abstract

Background: An ESBL, carbapenemase- and MCR-1-producing Escherichia coli ST648 strain was isolated from the urine sample of a patient in a Chinese tertiary hospital in 2016.

Methods: The strain was fully sequenced by GridION X5 platform of Oxford Nanopore Technology.

Results: The sequence analysis showed that the extended-spectrum β-lactamases CTX-M-65 and OXA-1, the carbapenemase NDM-5, the MCR-1 were encoded, respectively, by three different resistance plasmids. The pE648CTX-M-65-carrying bla _CTX-M-65 was a novel conjugative plasmid belonging to IncHI2 type; except for the bla _CTX-M-65, it also carried resistance genes ble, floR, sul1, aph(4)-Ia, aac(3)-VI, aac(6')-II, bla _OXA-1, catB, arr3 and tetA. Besides, an IncX4 plasmid pE648MCR-1-carrying mcr-1 and an IncX3 plasmid pE648NDM-5-carrying bla _NDM-5 were also identified.

Conclusion: The three transferable resistance plasmids coexisting in the E. coli ST648 isolate indicated the high risk to disseminate the extensively-drug-resistance among Enterobacteriaceae.

Keywords: CTX-M-65; Escherichia coli; MCR-1; NDM-5; extensively-drug-resistance.

Grants and funding

This work was supported by National Natural Science Foundation of China (No. 31372453) in the design of the research, sequencing and interpretation of data; and also supported by Six talent peaks project of Jiangsu Province (WSN-091) by sample collection and analysis.