Characterization of the intestinal microbiota during Citrobacter rodentium infection in a mouse model of infection-triggered Parkinson's disease

Gut Microbes. 2020 Nov 9;12(1):1-11. doi: 10.1080/19490976.2020.1830694.

Abstract

Parkinson's disease (PD) is a neurodegenerative disorder that has been shown to be influenced by the intestinal milieu. The gut microbiota is altered in PD patients, and murine studies have begun suggesting a causative role for the gut microbiota in progression of PD. We have previously shown that repeated infection with the intestinal murine pathogen Citrobacter rodentium resulted in the development of PD-like pathology in Pink1-/- mice compared to wild-type littermates. This addendum aims to expand this work by characterizing the gut microbiota during C. rodentium infection in our Pink1-/- PD model. We observed little disturbance to the fecal microbiota diversity both between infection timepoints and between Pink1-/- and wild-type control littermates. However, the level of short-chain fatty acids appeared to be altered over the course of infection with butyric acid significantly increasing in Pink1-/- mice and isobutyric acid increasing in wild-type mice.

Keywords: Gut microbiota; Parkinson’s disease; autoimmunity; gut-brain axis; infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteria / classification
  • Bacteria / growth & development
  • Citrobacter rodentium / physiology*
  • Disease Models, Animal
  • Enterobacteriaceae Infections / microbiology*
  • Fatty Acids, Volatile / analysis
  • Fatty Acids, Volatile / metabolism
  • Feces / chemistry
  • Feces / microbiology
  • Gastrointestinal Microbiome*
  • Mice
  • PTEN-Induced Putative Kinase
  • Parkinson Disease / microbiology*
  • Protein Kinases / genetics

Substances

  • Fatty Acids, Volatile
  • Protein Kinases
  • PTEN-Induced Putative Kinase