Toward Increased Reliability, Transparency, and Accessibility in Cross-linking Mass Spectrometry

Structure. 2020 Nov 3;28(11):1259-1268. doi: 10.1016/j.str.2020.09.011. Epub 2020 Oct 15.


Cross-linking mass spectrometry (MS) has substantially matured as a method over the past 2 decades through parallel development in multiple labs, demonstrating its applicability to protein structure determination, conformation analysis, and mapping protein interactions in complex mixtures. Cross-linking MS has become a much-appreciated and routinely applied tool, especially in structural biology. Therefore, it is timely that the community commits to the development of methodological and reporting standards. This white paper builds on an open process comprising a number of events at community conferences since 2015 and identifies aspects of Cross-linking MS for which guidelines should be developed as part of a Cross-linking MS standards initiative.

Publication types

  • Congress
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cross-Linking Reagents / chemistry*
  • Guidelines as Topic
  • Humans
  • International Cooperation
  • Mass Spectrometry / instrumentation
  • Mass Spectrometry / methods*
  • Mass Spectrometry / standards
  • Protein Conformation
  • Protein Interaction Mapping / methods
  • Proteins / ultrastructure*
  • Proteomics / instrumentation
  • Proteomics / methods*
  • Proteomics / standards
  • Reproducibility of Results


  • Cross-Linking Reagents
  • Proteins