Yeosuana marina sp. nov., isolated from shallow-sea hydrothermal systems off Kueishantao Island

Int J Syst Evol Microbiol. 2020 Dec;70(12):6251-6256. doi: 10.1099/ijsem.0.004525. Epub 2020 Oct 19.


A novel Gram-stain-negative, non-flagellated, non-motile, rod-shaped (0.4-0.6×1.8-2.5 µm), aerobic bacterial strain, designated JLT21T, was isolated from seawater of a shallow-sea hydrothermal system. Growth occurred with 0-4.0 % (w/v) NaCl (optimum, 2.0 % NaCl), at 8-45 °C (optimum, 25 °C) and at pH 3.0-10.0 (optimum, pH 7.0). Analysis of 16S rRNA gene sequences revealed that strain JLT21T showed the highest 16S rRNA gene sequence similarity to Gaetbulibacter aquiaggeris KEM-8T (97.2 %), Gaetbulibacter marinus IMCC1914T (96.9 %) and Yeosuana aromativorans GW1-1T (96.9 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JLT21T clustered with Y. aromativorans GW1-1T. The predominant respiratory quinone of strain JLT21T was menaquinone-6 (MK-6). Polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, three unidentified glycolipids and four unidentified lipids. The major fatty acids of strain JLT21T were iso-C15 : 0 (21.7 %), C16 : 1 ω6c/ω7c (11.5 %) and iso-C17 : 0 3-OH (10.9 %). The DNA G+C content of strain JLT21T was 32.6 %. On the basis of polyphasic analysis, strain JLT21T is considered to represent a novel species of the genus Yeosuana, for which the name Yeosuana marina sp. nov. is proposed. The type strain of Yeosuana marina is JLT21T (=CGMCC 1.15787T=JCM 31511T). The study helps us better understand the bacterial species in the shallow-sea hydrothermal system and their adaptations to the hydrothermal environment.

Keywords: Yeosuana; Yeosuana marina; phylogenetic analysis; shallow-sea hydrothermal system.

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Flavobacteriaceae / chemistry
  • Flavobacteriaceae / classification*
  • Flavobacteriaceae / genetics
  • Hydrothermal Vents / microbiology*
  • Islands
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Species Specificity


  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S

Supplementary concepts

  • Gaetbulibacter aquiaggeris
  • Gaetbulibacter marinus
  • Yeosuana aromativorans