Transcriptomic Analysis in the Sea Anemone Nematostella vectensis

Methods Mol Biol. 2021;2219:231-240. doi: 10.1007/978-1-0716-0974-3_14.

Abstract

The sea anemone Nematostella vectensis is an emerging research model to study embryonic development and regeneration at the molecular and global transcriptomic level. Transcriptomics analysis is now routinely used to detect differential expression at the genome level. Here we present the latest procedures for isolating high-quality RNA required for next generation sequencing, as well as methods and resources for quantifying transcriptomic data.

Keywords: Cnidarian; De novo assembly; Nematostella vectensis; RNA isolation; transcriptome.