Dual effect of 2D WS2 nanoparticles on the lysozyme conformation

I O Polovyi; O P Gnatyuk; K O Pyrshev; T O Hanulia; T P Doroshenko; S A Karakhim; O Yu Posudievsky; A S Kondratyuk; V G Koshechko; G I Dovbeshko

doi:10.1016/j.bbapap.2020.140556

Dual effect of 2D WS₂ nanoparticles on the lysozyme conformation

Biochim Biophys Acta Proteins Proteom. 2021 Jan;1869(1):140556. doi: 10.1016/j.bbapap.2020.140556. Epub 2020 Oct 17.

Authors

I O Polovyi¹, O P Gnatyuk², K O Pyrshev³, T O Hanulia⁴, T P Doroshenko⁵, S A Karakhim⁶, O Yu Posudievsky⁷, A S Kondratyuk⁷, V G Koshechko⁷, G I Dovbeshko²

Affiliations

¹ Institute of Physics of the National Academy of Sciences of Ukraine, Nauky avenue, 46, Kyiv 03028, Ukraine. Electronic address: ipoliovy@gmail.com.
² Institute of Physics of the National Academy of Sciences of Ukraine, Nauky avenue, 46, Kyiv 03028, Ukraine.
³ Institute of Physics of the National Academy of Sciences of Ukraine, Nauky avenue, 46, Kyiv 03028, Ukraine; O.V. Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Leontovycha street, 9, Kyiv 01054, Ukraine.
⁴ Institute of Physics of the National Academy of Sciences of Ukraine, Nauky avenue, 46, Kyiv 03028, Ukraine; Institute of Low Temperature and Structure Research of the Polish Academy of Sciences, Okolna street, 2, Wroclaw 50-422, Poland.
⁵ V. Lashkaryov Institute of Semiconductor Physics of the National Academy of Sciences of Ukraine, Nauky avenue, 41, Kyiv 03028, Ukraine.
⁶ O.V. Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Leontovycha street, 9, Kyiv 01054, Ukraine.
⁷ L.V. Pisarzhevsky Institute of Physical Chemistry of the National Academy of Sciences of Ukraine, Nauky avenue, 31, Kyiv 03028, Ukraine.

PMID: 33075478
DOI: 10.1016/j.bbapap.2020.140556

Abstract

In the present work we studied the effect of 2D WS₂ nanoparticles on the conformational changes in lysozyme protein at different pH values (2.0-11.5). The contributions of various structural conformations (α-helix, β-sheets parallel and antiparallel, unordered structure and side groups) were determined by decomposition of Amid I absorbance bands. The 2D WS₂ were shown to have different impact on secondary structure depending on pH of the solution and protein concentration. The amyloid fibril presence was confirmed with confocal microscopy enhanced by gold support, and fluorescent spectroscopy with amyloid-sensitive dye Thioflavin T. Our data show that WS₂ can both inhibit and stimulate amyloid formation. Additionally, we have also reported an unusual spectroscopic behavior displayed by lysozyme, indicated by narrowing of Amide I and Amide II bands at pH 2.5 and 3.5 when incubated with 2D WS2 nanoparticles.

Keywords: 2D WS2 particles; Amyloid fibrils; Fluorescent spectroscopy; IR spectroscopy; Lysozyme; α-Helix; β-Sheets (parallel and antiparallel).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amyloid / chemistry*
Animals
Benzothiazoles / chemistry
Chickens
Egg White / chemistry
Fluorescent Dyes / chemistry
Hydrogen-Ion Concentration
Metal Nanoparticles / chemistry*
Muramidase / chemistry*
Protein Conformation, alpha-Helical
Protein Conformation, beta-Strand
Spectrum Analysis / methods
Sulfides / chemistry*
Tungsten Compounds / chemistry*

Substances

Amyloid
Benzothiazoles
Fluorescent Dyes
Sulfides
Tungsten Compounds
tungsten sulfide
thioflavin T
Muramidase