Detecting and correlating bacterial populations to visual color change of polydiacetylene-coated filters

Yueyuan Zhang; Paul L Dawson; Timothy W Hanks; Julie K Northcutt; Tzuen-Rong Tzeng; William T Pennington

doi:10.1016/j.talanta.2020.121482

Detecting and correlating bacterial populations to visual color change of polydiacetylene-coated filters

Talanta. 2021 Jan 1:221:121482. doi: 10.1016/j.talanta.2020.121482. Epub 2020 Aug 5.

Authors

Yueyuan Zhang¹, Paul L Dawson², Timothy W Hanks³, Julie K Northcutt¹, Tzuen-Rong Tzeng⁴, William T Pennington⁵

Affiliations

¹ Department of Food, Nutrition, and Packaging Sciences, Clemson University, Clemson, SC, USA.
² Department of Food, Nutrition, and Packaging Sciences, Clemson University, Clemson, SC, USA. Electronic address: pdawson@clemson.edu.
³ Department of Chemistry, Furman University, Greenville, SC, USA.
⁴ Department of Biological Sciences, Clemson University, Clemson, SC, USA.
⁵ Department of Chemistry, Clemson University, Clemson, SC, USA.

PMID: 33076093
DOI: 10.1016/j.talanta.2020.121482

Abstract

Membrane filters were coated with 10,12-pentacosadiynoic acid (PCDA) then polymerized on the filter for rapid bacterial detection and quantification. The polymerized PCDA (pPDCA)-coated filter changed color in response to Salmonella Typhimurium and Escherichia coli but not to Listeria innocua. The time required for color change of pPCDA-coated filters was determined by a visual panel. A simple linear regression model was generated to fit the observed data and was validated with goodness of fit analysis and residual analysis. The pPCDA-filter method estimated Salmonella Typhimurium populations of 8 to 3 log CFU ml^-1 within 1.5-7.5 h, respectively.

Keywords: Biosensor; Escherichia coli; PCDA; Polydiacetylene; Salmonella typhimurium; Visual detection.

MeSH terms

Colony Count, Microbial
Food Microbiology
Listeria*
Polyacetylene Polymer

Substances

Polyacetylene Polymer
polydiacetylene

Supplementary concepts

Listeria innocua