Evaluation of cobas Influenza A/B & RSV Test for Diagnosis of Equine Influenza

J Equine Vet Sci. 2020 Nov;94:103249. doi: 10.1016/j.jevs.2020.103249. Epub 2020 Sep 12.

Abstract

A rapid and sensitive diagnostic method is needed to help prevent the spread of equine influenza virus. The cobas Influenza A/B & RSV test for the cobas Liat system (Roche Diagnostics) is based on real-time reverse transcription polymerase chain reaction and is designed to broadly detect influenza A virus RNA within 20 minutes. It detected a broad range of equine influenza virus strains, and detected equine influenza virus RNA from nasal swabs of infected horses at the same level as real-time reverse transcription polymerase chain reaction, although it returned some invalid results (7.7%). This suggests that cobas Influenza A/B & RSV test is highly sensitive to equine influenza virus, but should be improved to reduce the rate of invalid results.

Keywords: Diagnosis; Equine influenza virus; Real-time reverse transcription polymerase chain reaction; cobas liat system.

MeSH terms

  • Animals
  • Horse Diseases* / diagnosis
  • Horses
  • Influenza A virus* / genetics
  • Influenza B virus / genetics
  • Influenza, Human*
  • Molecular Diagnostic Techniques / veterinary
  • Sensitivity and Specificity