The 180-kD component of the neural cell adhesion molecule N-CAM is involved in cell-cell contacts and cytoskeleton-membrane interactions

Cell Tissue Res. 1987 Oct;250(1):227-36. doi: 10.1007/BF00214676.


N-CAM180, the molecular form of the three neural cell adhesion molecules (N-CAM) with the largest cytoplasmic domain, is accumulated at sites of cell-cell contact (cell bodies, neurites, growth cones) in cultures of neuroblastoma and cerebellum. At these sites the cytoskeleton-membrane linker protein brain spectrin and actin are also accumulated. Brain spectrin copurifies with N-CAM180 by immunoaffinity chromatography and binds specifically to N-CAM180 but not to N-CAM140 or N-CAM120 in a solid-phase binding test. These observations indicate an association of N-CAM180 with the cytoskeleton in vivo. This association may underlie the reduced lateral mobility of N-CAM180 in the surface membrane compared to N-CAM140 (Pollerberg et al. 1986). Together with the fact that N-CAM180 is only expressed after termination of neuron migration in vivo (Persohn and Schachner, unpublished) these results suggest a role for N-CAM180 in stabilization of cell contacts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / analysis*
  • Cell Adhesion
  • Cell Adhesion Molecules
  • Cell Communication*
  • Cell Line
  • Cell Membrane / ultrastructure*
  • Cells, Cultured
  • Cerebellum / cytology*
  • Cytoskeleton / ultrastructure*
  • Fluorescent Antibody Technique
  • Mice
  • Mice, Inbred Strains
  • Molecular Weight
  • Neuroblastoma


  • Antigens, Surface
  • Cell Adhesion Molecules