Cell-cycle-dependent expression of human ornithine decarboxylase

J Cell Physiol. 1987 Sep;132(3):545-51. doi: 10.1002/jcp.1041320318.


A human ornithine decarboxylase (ODC) gene probe has been isolated from a Jurkat T-cell cDNA expression library, sequenced, and used to analyze ODC mRNA levels in untransformed human lymphocytes and fibroblasts stimulated to proliferate by various mitogens. The partial cDNA sequence is 86% homologous to the mouse ODC cDNA, and Northern blots indicate that the human and mouse mRNA species are similar in size. ODC mRNA is barely detectable in quiescent human T lymphocytes and undetectable in density-arrested W138 fibroblasts. Following stimulation of T-lymphocyte proliferation with phytohemagglutinin, the ODC mRNA level rises to a peak around mid G1 phase and decreases as the cells enter S phase. Serum stimulation of density-arrested fibroblasts results in an elevation of the ODC mRNA level which persists throughout the cell cycle. Epidermal growth factor (20 ng/ml) but not insulin (10 mg/ml) or dexamethasone (55 ng/ml) stimulates ODC expression in quiescent W138 fibroblasts. Southern blots suggest that human cells have a single copy of the ODC gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Cycle*
  • DNA / metabolism
  • Dexamethasone / pharmacology
  • Epidermal Growth Factor / pharmacology
  • Fibroblasts / metabolism
  • Humans
  • Insulin / pharmacology
  • Mice
  • Molecular Sequence Data
  • Ornithine Decarboxylase / biosynthesis
  • Ornithine Decarboxylase / genetics*
  • RNA, Messenger / metabolism
  • T-Lymphocytes / metabolism


  • Insulin
  • RNA, Messenger
  • Epidermal Growth Factor
  • Dexamethasone
  • DNA
  • Ornithine Decarboxylase

Associated data

  • GENBANK/M20372