bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting MDFIC Gene

Genes (Basel). 2020 Oct 20;11(10):1232. doi: 10.3390/genes11101232.

Abstract

miR-23a, a member of the miR-23a/24-2/27a cluster, has been demonstrated to play pivotal roles in many cellular activities. However, the mechanisms of how bta-miR-23a controls the myogenic differentiation (MD) of PDGFRα- bovine progenitor cells (bPCs) remain poorly understood. In the present work, bta-miR-23a expression was increased during the MD of PDGFRα- bPCs. Moreover, bta-miR-23a overexpression significantly promoted the MD of PDGFRα- bPCs. Luciferase reporter assays showed that the 3'-UTR region of MDFIC (MyoD family inhibitor domain containing) could be a promising target of bta-miR-23a, which resulted in its post-transcriptional down-regulation. Additionally, the knockdown of MDFIC by siRNA facilitated the MD of PDGFRα- bPCs, while the overexpression of MDFIC inhibited the activating effect of bta-miR-23a during MD. Of note, MDFIC might function through the interaction between MyoG transcription factor and MEF2C promoter. This study reveals that bta-miR-23a can promote the MD of PDGFRα- bPCs through post-transcriptional downregulation of MDFIC.

Keywords: MDFIC; bta-miR-23a; fetal muscle development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cell Differentiation*
  • Gene Expression Regulation, Developmental*
  • MicroRNAs / genetics*
  • Muscle Development*
  • Muscle, Skeletal / cytology*
  • Muscle, Skeletal / metabolism
  • Myogenic Regulatory Factors / antagonists & inhibitors*
  • Stem Cells / cytology*
  • Stem Cells / metabolism

Substances

  • MicroRNAs
  • Myogenic Regulatory Factors