A hydrophilic magnetic MOF for the consecutive enrichment of exosomes and exosomal phosphopeptides

Chem Commun (Camb). 2020 Nov 12;56(90):13999-14002. doi: 10.1039/d0cc06147f.


Hydrophilic Fe3O4@PDA@UIO-66-NH2 is employed to consecutively enrich urinary exosomes and exosomal phosphopeptides through the strong bond between Zr and phosphate groups. The recovery of exosomes reached 95% and 707 phosphopeptides were identified from 255 phosphoproteins via LC-MS/MS. This work will make a great contribution to further functional analysis of exosomes.