Photobiomodulation Therapy Reduces the Inflammatory Process without Inhibiting Bone Deposition in Rats in an Extraction Model

Photobiomodul Photomed Laser Surg. 2020 Nov;38(11):673-678. doi: 10.1089/photob.2020.4844. Epub 2020 Oct 23.


Objective: We evaluated the influence of photobiomodulation therapy (PBMT) application during bone healing postexodontia in rats. Methods: We divided 84 male Wistar rats into a control group (CG), which received placebo treatment without PBMT, and a test group (TG), which was treated with PBMT. After exodontia, the animals were subjected to PBMT (TG) with an AsGaAI diode laser at 810 nm, 100 mW, 2 J, and 70 J/cm2 or placebo treatment (CG) every 72 h. After 1, 3, 7, 14, 21, and 28 days, the animals were weighed and euthanized to remove the left hemimandibles for radiographic (alveolar filling) and histomorphometric (inflammatory polymorphonuclear cell (PMN), mononuclear cell (MN), osteoclast (OC), and blood vessels counting) analysis. Statistic approach used two-way variance analysis followed by Bonferroni post hoc (p < 0.05, GraphPad Prism 5.0). Results: There was no significant difference in body mass variation (p = 0.828) and bone neoformation (p = 0.365) between the two groups, but the TG presented lower PMN (p < 0.001), MN (p < 0.001), and OC counts (p < 0.001) and higher blood vessels count (p < 0.001) throughout the repair process. Conclusions: PBMT attenuated the inflammatory process after exodontia without interfering with bone neoformation.

Keywords: oral; photobiomodulation therapy; surgery; tooth.

MeSH terms

  • Animals
  • Lasers, Semiconductor
  • Low-Level Light Therapy*
  • Male
  • Rats
  • Rats, Wistar