Aurantiacibacter rhizosphaerae sp. nov., isolated from a rhizosphere mudflat of a halophyte and proposal to reclassify Erythrobacter suaedae Lee et al. 2019. and Erythrobacter flavus Yoon et al. 2003 as Aurantiacibacter suaedae comb. nov. and Qipengyuania flava comb. nov., respectively

Int J Syst Evol Microbiol. 2020 Dec;70(12):6257-6265. doi: 10.1099/ijsem.0.004524.

Abstract

A marine alphaproteobacterium, designated as strain GH3-10T, was isolated from the rhizosphere mud of a halophyte (Suaeda japonica) collected at the seashore of Gangwha Island, Republic of Korea. The isolate was found to be Gram-stain-negative, strictly aerobic, catalase- and oxidase-positive, non-motile, short rods and produced orange-coloured colonies. The 16S rRNA gene- and whole genome-based phylogenetic analyses exhibited that strain GH3-10T belonged to the genus Aurantiacibacter and was most closely related to Aurantiacibacter atlanticus s21-N3T (98.7 % 16S rRNA gene sequence similarity) and Aurantiacibacter marinus KCTC 23554T (98.4 %). The major respiratory quinone was ubiquinone-10. The polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid and an unidentified lipid. The major fatty acids were C18 : 1 ω7c, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C18 : 1 ω7c 10-methyl. The DNA G+C content was 61.3 mol% (by genome). Average nucleotide identity and DNA-DNA relatedness values between the isolate and its phylogenetically closest relatives, together with phenotypic distinctness warranted the taxonomic description of a new species. On the basis of data obtained by a polyphasic approach, strain GH3-10T (=KCTC 62379T=JCM 32444T) represents a novel species of the genus Aurantiacibacter, for which the name Aurantiacibacter rhizosphaerae sp. nov. is proposed. According to phylogenetic coherence based on 16S rRNA genes and core genomes, it is also proposed that Erythrobacter suaedae Lee et al. 2019. and Erythrobacter flavus Yoon et al. 2003 be transferred to Aurantiacibacter suaedae comb. nov. and Qipengyuania flava comb. nov., respectively.

Keywords: Aurantiacibacter suaedae comb. nov.; Erythrobacter rhizosphaerae; Qipengyuania flava comb. nov.; Suaeda japonica; halophyte; rhizosphere mudflat.

MeSH terms

  • Alphaproteobacteria / classification*
  • Alphaproteobacteria / isolation & purification
  • Bacterial Typing Techniques
  • Base Composition
  • Chenopodiaceae / microbiology*
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Islands
  • Nucleic Acid Hybridization
  • Phospholipids / chemistry
  • Phylogeny*
  • Pigmentation
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Rhizosphere*
  • Salt-Tolerant Plants / microbiology
  • Sequence Analysis, DNA
  • Sphingomonadaceae / classification*
  • Sphingomonadaceae / isolation & purification
  • Ubiquinone / analogs & derivatives
  • Ubiquinone / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • coenzyme Q10

Supplementary concepts

  • Erythrobacter flavus