Microvascular effects of microneedles with subsequent histamin application in the skin prick test

Skin Res Technol. 2021 May;27(3):400-403. doi: 10.1111/srt.12969. Epub 2020 Oct 23.


Background: Since decades, histamin applications are routinely performed in skin prick tests using a lancet. However, this technique is associated with various drawbacks.

Materials and methods: In healthy human subjects, we investigated the effects of microneedle-enhanced histamin delivery (wheal size, erythema size) in the skin microvasculature using polarized light spectroscopy imaging (Tissue Viability imaging, TiVi). Histamin was applied on microneedle-pretreated skin or on -untreated skin, to assess the microvascular response in the local skin.

Results: In our results, histamin was delivered more rapidly into the skin after microneedle pretreatment compared to passive diffusion, visible as wheal and erythema.

Conclusion: The here presented technique might be useful for a personalized drug-testing system in the future.

Keywords: TiVi imaging; histamin; microneedles; skin prick test.

MeSH terms

  • Administration, Cutaneous
  • Drug Delivery Systems
  • Erythema
  • Humans
  • Needles
  • Skin Tests
  • Skin*
  • Urticaria*