Linking microbial community structure with molecular composition of dissolved organic matter during an industrial-scale composting

Abstract

This study explored the interactions between dissolved organic matter (DOM) composition and microbial community structure during an industrial-scale composting by Fourier transform ion cyclotron resonance mass spectrometry and 16S rRNA sequencing analysis. The results revealed that DOM from matured compost contained primarily lignins/carboxylic-rich alicyclic molecules (73.6%), the higher double bond equivalent (5.97) and aromaticity index (0.18), indicating that the molecular composition of DOM had changed substantially. Drastic changes in microbial community structure were also observed along with the DOM transformation process of composting. Network analysis further indicated that Caldicoprobacter, Bacillus, and Dechloromonas were associated with the most DOM subcategories. Caldicoprobacter could degrade carbohydrates, Bacillus accelerated the humification by transforming N-containing compounds, and Dechloromonas could degrade polycyclic aromatic hydrocarbons distributed in low O/C. These findings are helpful for understanding the molecular mechanisms of DOM transformation and humification of sludge composting.

Keywords: Dissolved organic matter (DOM); Electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS); Industrial-scale composting; Microbial community structure; Molecular composition.