Differential Distribution of Ca2+ Channel Subtypes at Retinofugal Synapses

Abstract

Retinofugal synapses serve as models for understanding how sensory signals from the periphery are relayed to the brain. Past studies have focused primarily on understanding the postsynaptic glutamatergic receptor subtypes involved in signal transmission, but the mechanisms underlying glutamate release at presynaptic retinal terminals remains largely unknown. Here we explored how different calcium (Ca²⁺) channel subtypes regulate glutamatergic excitatory synaptic transmission in two principal retinorecipient targets, the dorsal lateral geniculate nucleus (dLGN) and superior colliculus (SC) of the mouse. We used an in vitro slice preparation to record the synaptic responses of dLGN and SC neurons evoked by the electrical stimulation of optic tract (OT) fibers before and during the application of selective Ca²⁺ channel blockers. We found that synaptic responses to paired or repetitive OT stimulation were highly sensitive to extracellular levels of Ca²⁺ and to selective antagonists of voltage gated Ca²⁺ channels, indicating that these channels regulate the presynaptic release of glutamate at retinal synapses in both dLGN and SC. Bath application of selective Ca²⁺ channel blockers revealed that P/Q-type Ca²⁺ channels primarily operate to regulate glutamate release at retinal synapses in dLGN, while N-type Ca²⁺ channels dominate release in the SC.

Keywords: N-type Ca2+ channel; P/Q-type Ca2+ channel; dorsal lateral geniculate nucleus; retinal synapse; superior colliculus.