Synthesis and cellular location of the ten influenza polypeptides individually expressed by recombinant vaccinia viruses

Virology. 1987 Oct;160(2):336-45. doi: 10.1016/0042-6822(87)90004-3.

Abstract

A complete set of recombinant vaccinia viruses that express each of the influenza virus polypeptides has been constructed. PB1, PB2, PA, HA, NP, M1, and NS1 genes were derived from influenza virus A/PR/8/34, NA from influenza virus A/Cam/46, and M2 and NS2 genes from influenza virus A/Udorn/72. Cells infected with these recombinant viruses synthesize influenza polypeptides that are precipitable with specific antisera and that have electrophoretic mobilities similar to the corresponding influenza virus polypeptides. Indirect immunofluorescence studies have shown that HA, NA, and MS2 proteins migrate to the cell surface; PB2, PB1, PA, NP, and NS1 proteins migrate to the cell nucleus; and M1 and NS2 are distributed throughout the cell, although NS2 accumulates preferentially in nuclei. These transport processes occurred independently of other influenza polypeptides and are therefore attributable to the intrinsic properties of the influenza polypeptides themselves.

MeSH terms

  • Antigens, Viral / genetics
  • Cell Compartmentation
  • Cell Line
  • DNA, Recombinant
  • Fluorescent Antibody Technique
  • Genetic Vectors
  • Influenza A virus / genetics*
  • Vaccinia virus / genetics
  • Viral Proteins / biosynthesis
  • Viral Proteins / genetics*
  • Viral Proteins / metabolism

Substances

  • Antigens, Viral
  • DNA, Recombinant
  • Viral Proteins