Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach

PLoS One. 2020 Oct 26;15(10):e0229430. doi: 10.1371/journal.pone.0229430. eCollection 2020.

Abstract

Many compounds have the potential to harm pancreatic beta-cells; organochlorine pollutants belong to those compounds. In this work, we aimed to find markers of acute toxicity of p,p'-DDT exposure among proteins expressed in NES2Y human pancreatic beta-cells employing 2-D electrophoresis. We exposed NES2Y cells to a high concentration (150 μM, LC96 after 72 hours) of p,p'-DDT for 24 and 30 hours and determined proteins with changed expression using 2-D electrophoresis. We have found 22 proteins that changed their expression. They included proteins involved in ER stress (GRP78, and endoplasmin), mitochondrial proteins (GRP75, ECHM, IDH3A, NDUS1, and NDUS3), proteins involved in the maintenance of the cell morphology (EFHD2, TCPA, NDRG1, and ezrin), and some other proteins (HNRPF, HNRH1, K2C8, vimentin, PBDC1, EF2, PCNA, biliverdin reductase, G3BP1, FRIL, and HSP27). The proteins we have identified may serve as indicators of p,p'-DDT toxicity in beta-cells in future studies, including long-term exposure to environmentally relevant concentrations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / metabolism*
  • Cell Line
  • Cell Survival / drug effects
  • DDT / toxicity*
  • Electrophoresis, Gel, Two-Dimensional
  • Endoplasmic Reticulum Chaperone BiP
  • Gene Expression Regulation / drug effects
  • Humans
  • Insulin-Secreting Cells / cytology*
  • Insulin-Secreting Cells / drug effects
  • Insulin-Secreting Cells / metabolism
  • Mass Spectrometry
  • Proteomics / methods*

Substances

  • Biomarkers
  • Endoplasmic Reticulum Chaperone BiP
  • HSPA5 protein, human
  • DDT

Grant support

This work was supported by the research projects UNCE 204015 and PROGRES Q36 of Charles University in Prague, Czech Republic, and by the project BIOCEV CZ.1.05/1.1.00/02.0109 from the European Regional Development Fund. The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript.