New Gene Markers Involved in Molecular Processes of Tissue Repair, Response to Wounding and Regeneration Are Differently Expressed in Fibroblasts from Porcine Oral Mucosa during Long-Term Primary Culture

Artur Bryja; Patrycja Sujka-Kordowska; Aneta Konwerska; Sylwia Ciesiółka; Maria Wieczorkiewicz; Dorota Bukowska; Paweł Antosik; Rut Bryl; Mariusz T Skowroński; Jędrzej M Jaśkowski; Paul Mozdziak; Ana Angelova Volponi; Jamil A Shibli; Bartosz Kempisty; Marta Dyszkiewicz-Konwińska

doi:10.3390/ani10111938

New Gene Markers Involved in Molecular Processes of Tissue Repair, Response to Wounding and Regeneration Are Differently Expressed in Fibroblasts from Porcine Oral Mucosa during Long-Term Primary Culture

Animals (Basel). 2020 Oct 22;10(11):1938. doi: 10.3390/ani10111938.

Authors

Artur Bryja¹, Patrycja Sujka-Kordowska^{2

3}, Aneta Konwerska², Sylwia Ciesiółka², Maria Wieczorkiewicz⁴, Dorota Bukowska⁵, Paweł Antosik⁶, Rut Bryl¹, Mariusz T Skowroński⁴, Jędrzej M Jaśkowski⁵, Paul Mozdziak⁷, Ana Angelova Volponi⁸, Jamil A Shibli⁹, Bartosz Kempisty^{1

2

6

10}, Marta Dyszkiewicz-Konwińska^{1

11}

Affiliations

¹ Department of Anatomy, Poznan University of Medical Science, 60-781 Poznań, Poland.
² Department of Histology and Embryology, Poznan University of Medical Science, 60-781 Poznań, Poland.
³ Department of Anatomy and Histology, University of Zielona Gora, 65-046 Zielona Góra, Poland.
⁴ Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland.
⁵ Department of Diagnostics and Clinical Sciences, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland.
⁶ Department of Veterinary Surgery, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland.
⁷ Physiology Graduate Program, North Carolina State University, Raleigh, NC 27695, USA.
⁸ Department of Craniofacial Development and Stem Cell Biology, King's College University of London, London WC2R 2LS, UK.
⁹ Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos, Guarulhos SP 07030-010, Brazil.
¹⁰ Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 601 77 Brno, Czech Republic.
¹¹ Department of Biomaterials and Experimental Dentistry, Poznan University of Medical Sciences, 61-701 Poznań, Poland.

Abstract

The mechanisms of wound healing and vascularization are crucial steps of the complex morphological process of tissue reconstruction. In addition to epithelial cells, fibroblasts play an important role in this process. They are characterized by dynamic proliferation and they form the stroma for epithelial cells. In this study, we have used primary cultures of oral fibroblasts, obtained from porcine buccal mucosa. Cells were maintained long-term in in vitro conditions, in order to investigate the expression profile of the molecular markers involved in wound healing and vascularization. Based on the Affymetrix assays, we have observed three ontological groups of markers as wound healing group, response to wounding group and vascularization group, represented by different genes characterized by their expression profile during long-term primary in vitro culture (IVC) of porcine oral fibroblasts. Following the analysis of gene expression in three previously identified groups of genes, we have identified that transforming growth factor beta 1 (TGFB1), ITGB3, PDPN, and ETS1 are involved in all three processes, suggesting that these genes could be recognized as markers of repair specific for oral fibroblasts within the porcine mucosal tissue.

Keywords: fibroblasts; microarray; porcine; primary culture.