Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe

doi:10.1039/d0cc05900e

. 2020 Nov 12;56(90):14075-14078.

doi: 10.1039/d0cc05900e.

Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe

Jintao Zhao¹, Yuan Qu², Hao Gao², Miao Zhong¹, Xinming Li³, Fang Zhang¹, Yaxiong Chen⁴, Lu Gan⁵, Guodong Hu¹, Hong Zhang⁵, Shengxiang Zhang², Jianguo Fang¹

Affiliations

¹ State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China. fangjg@lzu.edu.cn.
² Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou 730000, China. sxzhang@lzu.edu.cn.
³ State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China. fangjg@lzu.edu.cn and State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
⁴ State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China. fangjg@lzu.edu.cn and Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000, China.
⁵ Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000, China.

PMID: 33107534
DOI: 10.1039/d0cc05900e

Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe

Jintao Zhao et al. Chem Commun (Camb). 2020.

. 2020 Nov 12;56(90):14075-14078.

doi: 10.1039/d0cc05900e.

Authors

Jintao Zhao¹, Yuan Qu², Hao Gao², Miao Zhong¹, Xinming Li³, Fang Zhang¹, Yaxiong Chen⁴, Lu Gan⁵, Guodong Hu¹, Hong Zhang⁵, Shengxiang Zhang², Jianguo Fang¹

Affiliations

¹ State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China. fangjg@lzu.edu.cn.
² Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou 730000, China. sxzhang@lzu.edu.cn.
³ State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China. fangjg@lzu.edu.cn and State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
⁴ State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China. fangjg@lzu.edu.cn and Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000, China.
⁵ Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000, China.

PMID: 33107534
DOI: 10.1039/d0cc05900e

Abstract

Thioredoxin reductase (TrxR) enzymes are critical in regulating redox homeostasis in cells. We report the first two-photon fluorescent probe of mammalian TrxR (TP-TRFS). TP-TRFS retains high specificity in recognizing TrxR. More importantly, the two-photon absorbing character of TP-TRFS enables it to be used in vivo. With the aid of TP-TRFS, a remarkable decline of the TrxR function was observed in the brain of a mouse model of stroke for the first time, providing a mechanistic link of TrxR dysfunction with stroke.

PubMed Disclaimer

Cited by

Fluorescent Probes for Mammalian Thioredoxin Reductase: Mechanistic Analysis, Construction Strategies, and Future Perspectives.
Song Z, Fan C, Zhao J, Wang L, Duan D, Shen T, Li X. Song Z, et al. Biosensors (Basel). 2023 Aug 13;13(8):811. doi: 10.3390/bios13080811. Biosensors (Basel). 2023. PMID: 37622897 Free PMC article. Review.
Potential enhancement of post-stroke angiogenic response by targeting the oligomeric aggregation of p53 protein.
Tam HH, Zhu D, Ho SSK, Vong HW, Wong VKW, Mok SW, Wong IN. Tam HH, et al. Front Cell Neurosci. 2023 Jul 18;17:1193362. doi: 10.3389/fncel.2023.1193362. eCollection 2023. Front Cell Neurosci. 2023. PMID: 37534043 Free PMC article. Review.
Recent Progress on NIR Fluorescent Probes for Enzymes.
Zhao J, Ma T, Chang B, Fang J. Zhao J, et al. Molecules. 2022 Sep 12;27(18):5922. doi: 10.3390/molecules27185922. Molecules. 2022. PMID: 36144654 Free PMC article. Review.
Cyclic 5-membered disulfides are not selective substrates of thioredoxin reductase, but are opened nonspecifically.
Felber JG, Poczka L, Scholzen KC, Zeisel L, Maier MS, Busker S, Theisen U, Brandstädter C, Becker K, Arnér ESJ, Thorn-Seshold J, Thorn-Seshold O. Felber JG, et al. Nat Commun. 2022 Apr 1;13(1):1754. doi: 10.1038/s41467-022-29136-4. Nat Commun. 2022. PMID: 35365603 Free PMC article.

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
- Royal Society of Chemistry
Medical
- MedlinePlus Health Information

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe

Affiliations

Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe

Authors

Affiliations

Abstract

Similar articles

Cited by

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Medical

Abstract

Similar articles

Cited by

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources

Medical