Generation of Engineered Human Myocardium in a Multi-well Format

STAR Protoc. 2020 Jun 3;1(1):100032. doi: 10.1016/j.xpro.2020.100032. eCollection 2020 Jun 19.

Abstract

This protocol describes a robust method for the generation of engineered human myocardium (EHM) from pluripotent stem cells (PSCs) in a multi-well plate under defined, serum-free conditions. By parallel culture of up to 48 EHM in one plate, contractile heart muscle can be obtained to serve numerous applications, including drug screening and disease modelling. This protocol has been successfully applied to human embryonic stem (HES) cell- and induced PSC-derived cardiomyocytes, subtype-specific, i.e., atrial and ventricular, and commercially available cardiomyocyte preparations. For complete details on the use and execution of this protocol, please refer to Tiburcy et al. (2017).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • Embryonic Stem Cells / cytology
  • Humans
  • Induced Pluripotent Stem Cells / cytology
  • Myocardium / cytology*
  • Myocytes, Cardiac / cytology*
  • Tissue Engineering / methods*