Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells

STAR Protoc. 2020 Aug 2;1(2):100078. doi: 10.1016/j.xpro.2020.100078. eCollection 2020 Sep 18.


Mammalian hematopoietic stem cells (HSCs) maintain life-long hematopoiesis in the bone marrow. HSCs remain quiescent in vivo, unlike more differentiated progenitors, and enter the cell cycle rapidly after bone marrow injury or in vitro culture. We have recently demonstrated the ability to maintain HSC quiescence in vitro by mimicking the bone marrow microenvironment. Here, we provide a detailed protocol for keeping functional HSCs in the quiescent state in vitro. For complete details on the use and execution of this protocol, please refer to Kobayashi et al. (2019).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / chemistry
  • Bone Marrow Cells / classification
  • Bone Marrow Cells / cytology
  • Cell Culture Techniques / methods*
  • Cell Proliferation
  • Cells, Cultured
  • Hematopoietic Stem Cells / chemistry
  • Hematopoietic Stem Cells / classification
  • Hematopoietic Stem Cells / cytology*
  • Mice