The Saccharomyces cerevisiae ABC subfamily D transporter Pxa1/Pxa2p co-imports CoASH into the peroxisome

FEBS Lett. 2021 Mar;595(6):763-772. doi: 10.1002/1873-3468.13974. Epub 2020 Nov 11.

Abstract

ATP-binding cassette (ABC) subfamily D transporters are important for the uptake of fatty acids and other beta-oxidation substrates into peroxisomes. Genetic and biochemical evidence indicates that the transporters accept fatty acyl-coenzyme A that is cleaved during the transport cycle and then re-esterified in the peroxisomal lumen. However, it is not known whether free coenzyme A (CoA) is released inside or outside the peroxisome. Here we have used Saccharomyces cerevisiae and isolated peroxisomes to demonstrate that free CoA is released in the peroxisomal lumen. Thus, ABC subfamily D transporter provide an import pathway for free CoA that controls peroxisomal CoA homeostasis and tunes metabolism according to the cell's demands.

Keywords: ABC transporter; acyl-CoA; coenzyme A; coenzyme homeostasis; peroxisome; thioesterase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • ATP-Binding Cassette Transporters / metabolism*
  • Acyl Coenzyme A / genetics
  • Acyl Coenzyme A / metabolism*
  • Biological Transport, Active
  • Peroxisomes / genetics
  • Peroxisomes / metabolism*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*

Substances

  • ATP-Binding Cassette Transporters
  • Acyl Coenzyme A
  • PXA1 protein, S cerevisiae
  • PXA2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins