A Multidrug-resistant Monophasic Salmonella Typhimurium Co-harboring mcr-1, fosA3, bla CTX-M-14 in a Transferable IncHI2 Plasmid from a Healthy Catering Worker in China

Zhenyu Wang; Haiyan Xu; Yuanyue Tang; Qiuchun Li; Xinan Jiao

doi:10.2147/IDR.S272272

A Multidrug-resistant Monophasic Salmonella Typhimurium Co-harboring mcr-1, fosA3, bla _CTX-M-14 in a Transferable IncHI2 Plasmid from a Healthy Catering Worker in China

Infect Drug Resist. 2020 Oct 13:13:3569-3574. doi: 10.2147/IDR.S272272. eCollection 2020.

Authors

Zhenyu Wang^#^{1

2

3}, Haiyan Xu^#⁴, Yuanyue Tang^{1

2

3}, Qiuchun Li^{1

2

3}, Xinan Jiao^{1

2

3}

Affiliations

¹ Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-Food Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, People's Republic of China.
² Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, People's Republic of China.
³ Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou, People's Republic of China.
⁴ Nantong Center for Disease Control and Prevention, Nantong, People's Republic of China.

^# Contributed equally.

Abstract

Background: Polymyxins are currently regarded as a possible last-resort therapy to eradicate multidrug-resistant (MDR) gram-negative bacteria. Meanwhile, the old antimicrobial agent fosfomycin has recently been reintroduced into clinical use for the treatment of extended-spectrum β-lactamase (ESBL)-producing and carbapenem-resistant Enterobacteriaceae. This study investigated a multidrug-resistant Salmonella 4,[5],12:i:- strain from a food catering handler, which had the potential to act as a vehicle for transmitting MDR foodborne pathogens.

Methods: A Salmonella 4,[5],12:i:- YZU1189 strain was isolated from the fecal sample of a food catering worker according to the standard protocol of the Salmonella detection method from World Health Organization in 2003. Serotyping of YZU1189 was performed according to the Kauffmann-White scheme. The antimicrobial resistance phenotype of the strain was determined by the agar dilution method according to the instruction from Clinical and Laboratory Standards Institute (CLSI). Plasmid conjugation was performed between the donor strain Salmonella 4,[5],12:i:- YZU1189 and the recipient strain Escherichia coli C600. The genetic locations of mcr-1, bla _CTX-M-14 and fosA3 genes were determined by the whole genome sequence analysis.

Results: Salmonella 4,[5],12:i:- YZU1189 was an ESBL-producing stain isolated from a healthy catering worker. The strain displayed resistance to aminoglycosides, beta-lactams, polymyxins, fosfomycins, phenicols, trimethoprims, sulfonamides, tetracyclines and fluoroquinolones. Whole genome sequence analysis and plasmid conjugation revealed that the strain had a transferable IncHI2 plasmid carrying the mcr-1, bla _CTX-M-14 and fosA3 genes. Sequence homology analysis showed that this plasmid possessed high sequence similarity to previously reported mcr-1, bla _CTX-M-14 and fosA3 positive plasmids in China.

Conclusion: This study reported a the multidrug-resistant Salmonella 4,[5],12:i:- isolate harboring mcr-1, bla _CTX-M-14 and fosA3 from human for the first time in China. The occurrence of mcr-1 and fosA3 genes in the transferable IncHI2 plasmid pYZU1189 from the ESBL-producing Salmonella 4,[5],12:i:- isolate showed a potential threat to public health. Great concern should be taken for the spread of multidrug-resistant ESBL-producing Salmonella isolates from food catering workers to consumers.

Keywords: ESBL; Salmonella 4,[5],12:i:-; colistin resistance; fosA3; multidrug resistant; whole genome sequencing analysis.

Grants and funding

This research was funded by National Natural Science Foundation of China, grant number 3192010301, and 31730094; The Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD) and Jiangsu Key Laboratory of Zoonosis (R1703).