Studies on the Sequence and Structure of the Escherichia Coli K-12 nupG Gene, Encoding a Nucleoside-Transport System

Eur J Biochem. 1987 Oct 15;168(2):385-91. doi: 10.1111/j.1432-1033.1987.tb13431.x.

Abstract

The nupG gene, encoding one of the two active nucleoside-transport systems in Escherichia coli K-12, has been cloned on the multicopy plasmid pBR322 and derivatives thereof. The recombinant plasmids complemented a chromosomal nupG mutation. A genetic map was determined by digestion with restriction endonucleases and the nucleotide sequence of a 3-kb stretch of DNA has been determined on fragments cloned into M13 phages. An open reading frame of 1254 bp, encoding a protein with a calculated molecular mass of 45.333 kDa, was deduced to be the coding region of nupG. Minicell-forming strains carrying plasmids containing this gene were shown to produce a hydrophobic, membrane-bound polypeptide with an apparent molecular mass of approximately 43 kDa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • Biological Transport, Active
  • Cloning, Molecular
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Genes*
  • Molecular Sequence Data
  • Nucleosides / metabolism*
  • Peptide Biosynthesis
  • Peptides / genetics
  • Plasmids
  • Promoter Regions, Genetic

Substances

  • Nucleosides
  • Peptides

Associated data

  • GENBANK/X06174