Cognitive Enhancer Noopept Activates Transcription Factor HIF-1

Dokl Biochem Biophys. 2020 Sep;494(1):256-260. doi: 10.1134/S1607672920050129. Epub 2020 Oct 20.


The effect of noopept (N-phenylacetyl-L-prolyl-glycine ethyl ester) on the DNA-binding activity of HIF-1 in SH-SH5Y cells and the mechanisms of stabilization of this transcription factor were studied in vitro. Noopept was shown to increase both the basal DNA-binding activity of HIF-1 and the activity induced by various hypoxia mimetics. The mechanism of stabilization of the oxygen-sensitive HIF1α subunit by noopept involves the inhibition of HIF-1 prolyl hydroxylase, which is indirectly indicated by the data obtained using the ODD-Luc reporter, and the positive effect on the level of the HIF1α protein. It was revealed that the effect of noopept is accompanied by changes in gene expression, which belong to different metabolic pathways and are controlled by the transcription factor HIF-1.

Keywords: FIH; HIF-1; HIF-1α stabilization; Pro-Gly dipeptides; hypoxia; noopept.

MeSH terms

  • Cell Line, Tumor
  • Dipeptides / pharmacology*
  • Humans
  • Hypoxia-Inducible Factor 1, alpha Subunit / metabolism*
  • Hypoxia-Inducible Factor-Proline Dioxygenases / metabolism*
  • Neuroblastoma / drug therapy
  • Neuroblastoma / metabolism*
  • Neuroblastoma / pathology
  • Neuroprotective Agents / pharmacology
  • Transcription Factors / metabolism*


  • Dipeptides
  • HIF1A protein, human
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Neuroprotective Agents
  • Transcription Factors
  • ethyl phenylacetyl-Pro-Gly
  • Hypoxia-Inducible Factor-Proline Dioxygenases