Increased Plasma Heparanase Activity in COVID-19 Patients

Front Immunol. 2020 Oct 6:11:575047. doi: 10.3389/fimmu.2020.575047. eCollection 2020.

Abstract

Reports suggest a role of endothelial dysfunction and loss of endothelial barrier function in COVID-19. It is well established that the endothelial glycocalyx-degrading enzyme heparanase contributes to vascular leakage and inflammation. Low molecular weight heparins (LMWH) serve as an inhibitor of heparanase. We hypothesize that heparanase contributes to the pathogenesis of COVID-19, and that heparanase may be inhibited by LMWH. To test this hypothesis, heparanase activity and heparan sulfate levels were measured in plasma of healthy controls (n = 10) and COVID-19 patients (n = 48). Plasma heparanase activity and heparan sulfate levels were significantly elevated in COVID-19 patients. Heparanase activity was associated with disease severity including the need for intensive care, lactate dehydrogenase levels, and creatinine levels. Use of prophylactic LMWH in non-ICU patients was associated with a reduced heparanase activity. Since there is no other clinically applied heparanase inhibitor currently available, therapeutic treatment of COVID-19 patients with low molecular weight heparins should be explored.

Keywords: COVID-19; LMWH (low molecular weight heparin); glycocalyx damage; heparanase; inflammation; vascular leakage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Betacoronavirus
  • COVID-19
  • Coronavirus Infections / immunology
  • Coronavirus Infections / pathology
  • Creatinine / blood
  • Critical Care
  • Cross-Sectional Studies
  • Endothelium / pathology*
  • Female
  • Glucuronidase / antagonists & inhibitors*
  • Glucuronidase / blood*
  • Glucuronidase / metabolism
  • Heparin Antagonists / therapeutic use*
  • Heparin, Low-Molecular-Weight / therapeutic use*
  • Heparitin Sulfate / blood
  • Humans
  • Interleukin-6 / blood
  • L-Lactate Dehydrogenase / blood
  • Male
  • Middle Aged
  • Pandemics
  • Pneumonia, Viral / immunology
  • Pneumonia, Viral / pathology
  • SARS-CoV-2
  • Tight Junctions / pathology*

Substances

  • Heparin Antagonists
  • Heparin, Low-Molecular-Weight
  • IL6 protein, human
  • Interleukin-6
  • Heparitin Sulfate
  • Creatinine
  • L-Lactate Dehydrogenase
  • heparanase
  • Glucuronidase