Production and characterization of monoclonal antibodies to 17 alpha-hydroxyprogesterone

J Steroid Biochem. 1987 Oct;28(4):405-10. doi: 10.1016/0022-4731(87)91058-2.


Hybridoma clones producing antibodies to 17 alpha-hydroxyprogesterone (17-OHP) were established by using a 17-OHP-bovine serum albumin conjugate as an immunogen. Six representative IgG-class monoclonal antibodies of high affinity (10(8)-10(9) M-1) showed differential reactivities with several structurally related steroids. Two enzyme immunoassay (EIA) systems (fluorescence EIA and micro-EIA) for 17-OHP using OHP 4B2.2.3, which showed the lowest cross-reactivity with other steroids, were established. The micro-EIA system was shown to be applicable to the mass-screening of congenital adrenal hyperplasia.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 17-alpha-Hydroxyprogesterone
  • Adrenal Hyperplasia, Congenital / blood
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • Antigens / immunology
  • Fluorescent Antibody Technique
  • Hybridomas / immunology
  • Hydroxyprogesterones / blood
  • Hydroxyprogesterones / immunology*
  • Immunoenzyme Techniques
  • Immunoglobulin G / immunology
  • Mass Screening
  • Mice
  • Mice, Inbred BALB C
  • Serum Albumin, Bovine / immunology


  • Antibodies, Monoclonal
  • Antigens
  • Hydroxyprogesterones
  • Immunoglobulin G
  • Serum Albumin, Bovine
  • 17-alpha-Hydroxyprogesterone