Description of Raphidascaris (Ichthyascaris) multipapillata sp. nov., Redescription of Hysterothylacium simile Li et al., 2013 and New Records of Some Other Ascaridoid and Seuratoid Nematodes from Marine Fishes in Japan

Acta Parasitol. 2021 Jun;66(2):461-474. doi: 10.1007/s11686-020-00299-8. Epub 2020 Nov 3.

Abstract

Purpose: Many nematode species parasitizing marine fishes were reported from Japanese waters. However, considering the wealth of fish species in this zoogeographically interesting region and the host specificity of nematodes, the number of hitherto recorded fish nematodes probably represents only a very small part of the potential Japanese fauna of these organisms. Therefore, new data are needed to recognise the species composition of these parasites in this region.

Methods: Helminthological examinations of some marine fishes from off Japan, carried out in the years 2005-2017, revealed one new and five insufficiently known species of ascaridoid and seuratoid nematodes. These were studied with the use of light and scanning electron microscopy.

Results: All six nematode species recorded are described: Anisakidae: Raphidascaris (Ichthyascaris) multipapillata sp. nov. (male and females) from the intestine of Labracoglossa argenteiventris Peters (Kyphosidae) in the Sea of Japan, Raphidascaris (Ichthyascaris) sp. (third-stage larvae) probably from the digestive tract of Seriola dumerili (Risso) and S. rivoliana Valenciennes (both Carangidae) in the western North Pacific Ocean off Ishigaki-jima Island, Okinawa Prefecture, Hysterothylacium simile Li et al., 2013 (males and females) from the stomach of Lateolabrax japonicus (Cuvier) (Lateolabracidae) in the Sea of Japan, Hysterothylacium sp. 1 (female) from the pyloric caeca of Jaydia lineata (Temminck et Schlegel) (Apogonidae) in the Seto Inland Sea, Hysterothylacium sp. 2 (third- and fourth-stage larvae) from the intestine and mesentery of Etelis coruscans Valenciennes (Lutjanidae) and Pseudorhombus pentophthalmus Günther (Paralichthyidae) in the western North Pacific Ocean off Ishigaki-jima Island, Okinawa Prefecture and the Seto Inland Sea, respectively; and Cucullanidae: Cucullanus sp. (female) from the intestine of Semicossyphus reticulatus (Valenciennes) (Labridae) in the Seto Inland Sea. Raphidascaris (I.) multipapillata sp. n. differs from congeners mainly in the high number (79) of pairs of caudal papillae, spicules 402 µm long and in the structure of the male and female tail tips. Hysterothylacium simile is redescribed, being recorded from off Japan for the first time.

Conclusions: In addition to the discovery of the new nematode species R. (I.) multipapillata sp. nov. and a redescription of H. simile providing some new morphological data of this parasite, the findings of all other species represent new host and geographical records. The present study extends the knowledge of the species composition and host-parasite relationships of nematodes parasitizing marine fishes.

Keywords: Cucullanus; East Asia; Hysterothylacium; Osteichthyes; Parasitic nematode.