The smfBox is an open-source platform for single-molecule FRET

Nat Commun. 2020 Nov 6;11(1):5641. doi: 10.1038/s41467-020-19468-4.


Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET's broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.

Publication types

  • Research Support, Non-U.S. Gov't