Association between genetic polymorphisms of telomere pathway genes and hydrogen peroxide level in omethoate exposure workers

Abstract

Objective: The aim of this study was to explore the association between genetic variations in telomere pathway genes and the level of hydrogen peroxide (H₂O₂) in omethoate exposure workers.

Methods: A total of 180 omethoate exposure workers and 115 healthy controls were recruited. The level of H₂O₂ in plasma was determined with molybdenic acid colorimetry. Polymerase chain reaction and restriction fragment length was used to detect polymorphisms in POT1 rs1034794, POT1 rs10250202, TERF1 rs3863242, and TERT rs2736098.

Results: The level of H₂O₂ in exposure group (4.26 ± 0.71) was significantly higher than that in control group (3.29 ± 0.46). Generalized linear models indicated that risk factors for the increase H₂O₂ level were exposure [β(95 % CI) = 0.951 (0.806, 1.096), P < 0.001] and AA + AT genotype in POT1 rs034794 [β(95 % CI) = 0.397 (0.049, 0.745), P = 0.025].

Conclusion: The increase H₂O₂ level was associated with omethoate exposure and AA + AT genotypes in POT1 gene rs1034794. It provided a new idea that polymorphisms in telomere pathway genes may indirectly regulate telomere length by influencing oxidative stress.

Keywords: Hydrogen peroxide; Omethoate; POT1; Polymorphisms; Rs1034794.