Evaluation of the neuroprotective efficiency of sodium hydrosulfide in neonatal rats with the induced hypoxic-ischemic encephalopathy model

Bratisl Lek Listy. 2020;121(11):789-795. doi: 10.4149/BLL_2020_129.


Aims: Hypoxic ischemic encephalopathy is one of the main causes of neonatal deaths. The objective of this study was to evaluate the neuroprotective effect of antioxidant and anti-inflammatory properties of sodium hydrosulfide (NaHS) in neonatal rats with hypoxic ischemic encephalopathy, as well as its effect on neuronal apoptosis through histopathological and biochemical tests.

Methods: Forty-seven-day‑old rats with induced hypoxia‑ischemia (HI) were randomly separated into four groups. Half an hour after the induction of hypoxic-ischemia, serum physiological (SF), 50 µmol/kg NaHS, or 100 µmol/kg NaHS were intraperitoneally given to the rats.

Results: Apoptotic cells in the brain tissue of rats in HI + NaHS 50 μmol/kg, and HI + NaHS 100 μmol/kg groups decreased compared to HI group (p = 0.00). While HI + NaHS 50 μmol/kg and HI + NaHS 100 μmol/kg groups yielded no difference in TNF-α, IL-6, and iNOS levels as compared to the HI group, an increase in NGF was detected in the 50 µmol/kg and 100 µmol/kg NaHS groups (p = 0.34, p = 0.24, p = 0.26, p = 0.026, p = 0.017). When TOS, TAS and OSI levels were compared, an increase in TAS and OSI and a decrease in TOS were observed in the treatment groups as compared to HI group.

Conclusions: NaHS given to hypoxic-ischemic encephalopathy model significantly decreased apoptosis in neurons and had a neuroprotective efficacy with an increase in NGF levels (Tab. 1, Fig. 3, Ref. 25).

Keywords: NGF antioxidant.; NaHS; hypoxic-ischemic encephalopathy; neonatal.

MeSH terms

  • Animals
  • Animals, Newborn
  • Apoptosis
  • Hypoxia-Ischemia, Brain* / drug therapy
  • Neurons / drug effects
  • Neuroprotective Agents* / pharmacology
  • Rats
  • Sulfides / pharmacology*


  • Neuroprotective Agents
  • Sulfides
  • sodium bisulfide