Chitosan Oligosaccharides Suppress Nuclear Factor-Kappa B Activation and Ameliorate Experimental Autoimmune Uveoretinitis in Mice

Int J Mol Sci. 2020 Nov 6;21(21):8326. doi: 10.3390/ijms21218326.

Abstract

We investigated the therapeutic potential and mechanism of chitosan oligosaccharides (COS) for experimental autoimmune uveoretinitis (EAU) in mice. EAU was induced in C57/BL6 mice by injection of human interphotoreceptor retinoid-binding protein (IRBP) peptides. At the same time, a high or low dose (20 or 10 mg/kg) of COS or phosphate-buffered saline (PBS) was given to mice daily after EAU induction. We found that mouse EAU is ameliorated by the high-dose COS treatment when compared with PBS treatment. In the retinas of high-dose COS-treated mice, the nuclear translocation of NF-κB subunit (p65) was suppressed, and the expression of several key EAU inflammatory mediators, IFN-γ, TNF-α, IL-1α, IL-4, IL-5, IL-6, IL-10, IL-17 and MCP-1 was lowered. These results suggest that COS may be a potential treatment for posterior uveitis.

Keywords: chitosan oligosaccharides (COS); experimental autoimmune uveoretinitis (EAU); nuclear factor-kappa B (NF-κB).

MeSH terms

  • Animals
  • Autoimmune Diseases / drug therapy
  • Autoimmune Diseases / immunology
  • Chitosan / metabolism
  • Chitosan / pharmacology*
  • Disease Models, Animal
  • Eye Proteins / adverse effects
  • Eye Proteins / metabolism
  • Female
  • Inflammation / metabolism
  • Interleukin-17 / immunology
  • Lymphocyte Activation / immunology
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B / metabolism*
  • Oligosaccharides / therapeutic use
  • Retina / metabolism
  • Retinitis / drug therapy*
  • Retinol-Binding Proteins / adverse effects
  • Retinol-Binding Proteins / metabolism
  • Tumor Necrosis Factor-alpha / immunology
  • Uveitis / drug therapy
  • Uveitis / metabolism

Substances

  • Eye Proteins
  • Interleukin-17
  • NF-kappa B
  • Oligosaccharides
  • Retinol-Binding Proteins
  • Tumor Necrosis Factor-alpha
  • interstitial retinol-binding protein
  • Chitosan