Metabolomic Analysis of Natural Variation in Arabidopsis

Methods Mol Biol. 2021;2200:393-411. doi: 10.1007/978-1-0716-0880-7_19.

Abstract

Methodological advances in coupled-mass spectrometry (gas chromatography and liquid chromatography; GC-MS and LC-MS) have rendered the profiling of highly complex plant extracts relatively facile and allowed that their high-throughput use aids the investigation of a range of biological questions. Among these is the elucidation of the genetic factors underlying metabolite abundance. For this purpose genome-wide association studies (GWAS) are being widely adopted in Arabidopsis with the resultant quantitative trait loci being subjected to cross-validation by the use of recombinant inbred lines, introgression lines, and T-DNA insertional knockout lines.

Keywords: Gas-chromatography mass-spectrometry; Genome-wide association mapping; Liquid-chromatography mass-spectrometry; Metabolomics; QTL analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis* / genetics
  • Arabidopsis* / metabolism
  • Gas Chromatography-Mass Spectrometry*
  • Genetic Variation*
  • Genome-Wide Association Study
  • Metabolomics*
  • Quantitative Trait Loci*