Protective effects of honey and bee venom against lipopolysaccharide and carbon tetrachloride-induced hepatoxicity and lipid peroxidation in rats

Noha M Meligi; Suzan Alaa Ismail; Nagy S Tawfik

doi:10.1093/toxres/tfaa077

Protective effects of honey and bee venom against lipopolysaccharide and carbon tetrachloride-induced hepatoxicity and lipid peroxidation in rats

Toxicol Res (Camb). 2020 Oct 15;9(5):693-705. doi: 10.1093/toxres/tfaa077. eCollection 2020 Sep.

Authors

Noha M Meligi¹, Suzan Alaa Ismail¹, Nagy S Tawfik¹

Affiliation

¹ Zoology Department, Faculty of Science, Minia University 61519, Minia, Egypt.

Abstract

In the present study, the protective effects of honey and bee venom (BV) either independently or in combination against lipopolysaccharide (LPS) and carbon tetrachloride (CCl₄)-induced hepatoxicity, lipid peroxidation, and hematological alterations in male albino rats were investigated. In addition, histopathological alterations of hepatic tissues induced by LPS/CCL₄ were recorded. Sixty-four of male albino rats of average weight 120-150 g were included in this study. Rats were divided into eight equal groups of eight. The obtained results demonstrated that treatment with LPS/CCl₄ caused an increase in the levels of alpha-fetoprotein, which was accompanied by changes in the hepatic function biomarkers that characterized by the increased levels of transaminases (AST, ALT). The results showed oxidative stress as assigned by the increase in lipid peroxide. Meantime detraction in the antioxidants, including glutathione peroxidase was observed. Interruptions in biochemical parameters accompanied by disturbances in hematological parameters and liver histopathology were resulted due to exposure to LPS/CCl₄. This study showed the use of honey and BV provided a protective effect on hepatotoxicity induced by LPS/CCl₄. This might have been occurred through the reduction of hepatic transaminases and the "Alpha-fetoprotein" in serum and the equilibration of the antioxidation system, thereby, inhibiting the reactive oxygen species accumulation. Honey and BV administration reestablish disturbed hematological parameters and liver histopathology persuaded by LPS/CCl₄. More interesting, we demonstrated that using a combination of the honey and BV showed promising enhancement in their protective effects over the use of just one of the two reagents.

Keywords: albino rats; bee venom; biochemical parameters; carbon tetrachloride (CCl4); hematological parameters; honey; lipopolysaccharide (LPS); liver.