Association of CD40 rs1883832 Polymorphism with Susceptibility of Diabetic Nephropathy and Neuropathy in Egyptian Population

Abstract

Diabetic nephropathy (DN) and peripheral neuropathy (DPN) are unpredictable diabetic complications with narrow management alternatives. CD40-CD40 ligand system may be an essential pathway for diabetic microvascular complications. No previous studies had evaluated the relationship between CD40 rs1883832 polymorphism and DN/DPN. This study aimed to investigate the association between CD40 rs1883832 polymorphism and the risk of nephropathy and neuropathy in Egyptian patients with type 2 diabetes mellitus. A total of 106 diabetic patients (53 with nephropathy and 53 with neuropathy) and 53 healthy controls (without DM or other overt chronic conditions) were recruited from Suez Canal University Hospitals. Genotyping of CD40 gene polymorphisms was carried out using the polymerase chain reaction-restriction fragment length polymorphism assay. Patients with TT genotype and T allele carry a higher risk of developing DN (odds ratio (OR)=5.40, P=0.0026) and OR=2.56, P=0.0009, respectively). Likewise, the risk of DPN was significantly higher in patients carrying TT genotype (OR=2.91, P=0.045) and T allele (OR=1.84, P=0.028), respectively. In conclusions, the T allele is significantly associated with DN and DPN. Further studies with larger sample sizes are necessary to confirm our observations.