Analysis of the genomic L RNA segment from lymphocytic choriomeningitis virus

Virology. 1987 Dec;161(2):448-56. doi: 10.1016/0042-6822(87)90138-3.


The arenavirus genomic L RNA segment represents approximately 70% of the viral genetic material but current understanding of the organization, regulation, and functioning of the viral L products remains limited. Biological studies with reassortant viruses have implicated the L RNA segment in the lethal infection of adult guinea pigs produced by LCMV-WE but no further explanation of the pathogenic process is presently available. We have initiated a detailed molecular analysis of LCMV L products based on construction and characterization of L-specific cDNA clones and synthesis of L-specific hybridization probes. Nucleotide sequencing studies have allowed the derivation of a partial amino acid sequence for a predicted L protein and antisera raised against synthetic peptides have demonstrated an L protein in Western blotting experiments. Using this approach we have identified a single high molecular weight protein (approximately 200,000 Da) in purified virions and in viral ribonucleoprotein complexes extracted from acutely infected tissue culture cells. This L protein is translated from a nonpolyadenylated, genomic complementary L mRNA and potentially represents part or all of the viral RNA-dependent RNA polymerase.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Genes, Viral
  • Immunosorbent Techniques
  • Lymphocytic choriomeningitis virus / genetics*
  • Molecular Sequence Data
  • RNA, Viral / genetics*
  • Sequence Homology, Nucleic Acid
  • Viral Proteins / genetics*
  • Viral Proteins / immunology


  • RNA, Viral
  • Viral Proteins
  • DNA

Associated data

  • GENBANK/M18381
  • GENBANK/M18382
  • GENBANK/M18383