Full-length transcriptome analysis of Misgurnus anguillicaudatus

Wei Luo; Qing Wu; Tianzhu Wang; Zhou Xu; Dongjie Wang; Yan Wang; Song Yang; Yuejin Long; Zongjun Du

doi:10.1016/j.margen.2020.100785

Full-length transcriptome analysis of Misgurnus anguillicaudatus

Mar Genomics. 2020 Dec:54:100785. doi: 10.1016/j.margen.2020.100785. Epub 2020 May 26.

Authors

Wei Luo¹, Qing Wu¹, Tianzhu Wang¹, Zhou Xu¹, Dongjie Wang¹, Yan Wang¹, Song Yang¹, Yuejin Long², Zongjun Du³

Affiliations

¹ College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
² ZiyangYanjiang District Agricultural Bureau, Ziyang 641300, China.
³ College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: 14364@sicau.edu.cn.

PMID: 33222891
DOI: 10.1016/j.margen.2020.100785

Abstract

Faithful analysis of transcripts represents a critical component of the gene discovery, genomic characterization and annotation process in species without a reference genome. The present study generated 28,001 full-length transcripts of Misgurnus anguillicaudatus using the Pacific Bioscience (PacBio) single-molecule real-time (SMRT) sequencing system. A total of 77,346 consensus isoforms were identified, and 18,991 complete open reading frames (ORFs) detected. In total, 1345 lncRNAs were identified with high-confidence, with additional identification of a number of well-known transcription factors. The present study may facilitate additional exploration of the genomic signatures of M. anguillicaudatus.

Keywords: Full-length transcripts; Misgurnus anguillicaudatus; PacBio SMRT.

MeSH terms

Animals
Cypriniformes / growth & development*
Female
Gene Expression Profiling / veterinary
Genome*
Male
Open Reading Frames / genetics
RNA, Long Noncoding / genetics
Transcriptome*

Substances

RNA, Long Noncoding