In this study, gold nanoparticles (AuNPs) were synthesised using seed extract of mango (Mangifera indica) which is considered as waste and generally thrown away into the environment. The bioactive molecules in the seed act as reducing agent to synthesise AuNPs without using any external agent. The characterisation of green synthesised AuNPs was done using various spectroscopic techniques. Visual colour change from colourless to ruby red colour confirmed the formation of AuNPs which was further confirmed by maximum absorption peak at 550 nm by UV-spectra. Crystalline nature was confirmed by XRD technique while round, triangle and irregular shape and 19.45 nm size was confirmed by TEM and SAED analysis. FTIR analysis confirmed the presence of alcohol or phenol, carboxylic acid, ketones, amines, aromatic amines, aliphatic amines, alkyl halides and alkynes in M. indica seed which were responsible for the reduction of gold to AuNPs. The green synthesised AuNPs were evaluated for their antimicrobial, antioxidant and cytotoxic potential. They showed moderate antibacterial, cytotoxic and dose-dependent antioxidant activity. Seeds of M. indica instead of discarding can be successfully utilised for AuNPs synthesis which can be used as a natural source of antimicrobial, antioxidant and anticancer agent. Highlights Green synthesis of gold nanoparticles (AuNPs) from fruit (Mangifera indica) waste (seed). Characterisation using various spectroscopic techniques: UV-Vis spectroscopy, Zeta potential, FTIR, XRD and TEM analysis. Synthesized AuNPs were round, triangle and irregular in shape and 19.45 nm in size. Antimicrobial activity of AuNPs against 14 microorganisms. Antioxidant activity of AuNPs in terms of DPPH, SO and ABTS. Cytotoxic activity against HeLa, MCF-7 and fibroblast normal cell lines.
Keywords: Mangifera indica; Green synthesis; anticancer; antimicrobial; antioxidant; biowaste; gold nanoparticles; seed extract.