Abstract
We studied the effect of KE and AED peptides on the expression of sirtuin-1, sirtuin-6, collagen I, cytokines (IL-1, TGF-β), and transcription factor NF-κB in human skin fibroblasts during their replicative aging. Immunocytochemical analysis and confocal microscopy showed that KE peptide reduces the synthesis of factors of the inflammatory response IL-1, NF-κB, and TGF-β and stimulates the synthesis of sirtuin-6. KE peptide normalizes the immunological function of human skin fibroblasts during their aging. AED peptide activates the synthesis of sirtuin-1, sirtuin-6, and collagen I in human skin fibroblasts during their replicative aging, which attests to its geroprotective effect.
Keywords:
collagen I; human skin fibroblasts; replicative aging; short peptides; sirtuins.
MeSH terms
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Antioxidants / chemical synthesis
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Antioxidants / pharmacology*
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Cellular Senescence / drug effects
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Collagen Type I / genetics
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Collagen Type I / immunology
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Female
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Fibroblasts / cytology
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Fibroblasts / drug effects*
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Fibroblasts / immunology
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Gene Expression Regulation / drug effects*
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Humans
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Immunologic Factors / chemical synthesis
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Immunologic Factors / pharmacology*
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Interleukin-1 / genetics
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Interleukin-1 / immunology
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NF-kappa B / genetics
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NF-kappa B / immunology
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Oligopeptides / chemical synthesis
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Oligopeptides / pharmacology*
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Primary Cell Culture
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Signal Transduction
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Sirtuin 1 / genetics
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Sirtuin 1 / immunology
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Sirtuins / genetics
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Sirtuins / immunology
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Transforming Growth Factor beta / genetics
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Transforming Growth Factor beta / immunology
Substances
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Antioxidants
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Collagen Type I
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Immunologic Factors
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Interleukin-1
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NF-kappa B
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Oligopeptides
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Transforming Growth Factor beta
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SIRT1 protein, human
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SIRT6 protein, human
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Sirtuin 1
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Sirtuins