Isolation of poly-alpha-L-guluronate Lyase From Klebsiella Aerogenes

Carbohydr Res. 1977 Aug;57:163-71. doi: 10.1016/s0008-6215(00)81928-x.

Abstract

The bacterium Klebsiella aerogenes type 25 produces an extracellular alginolyase which has been partly purified. The enzyme is specific for the alpha-L-guluronosyl linkages in whole alginate and fractions therefrom. The end products of its action on polyguluronic acid blocks are mainly the unsaturated di- and tri-saccharides, with a smaller proportion of the homologous tetrasaccharide. Some general properties of the enzyme are reported.

MeSH terms

  • Alginates
  • Cations
  • Hexuronic Acids
  • Klebsiella pneumoniae / enzymology*
  • Polysaccharide-Lyases* / isolation & purification
  • Polysaccharide-Lyases* / metabolism
  • Substrate Specificity

Substances

  • Alginates
  • Cations
  • Hexuronic Acids
  • Polysaccharide-Lyases
  • poly-alpha-L-guluronate lyase